Description
-
About MOTS-c (Mitochondrial ORF of the Twelve S rRNA type-c)
MOTS-c — Mitochondrial ORF of the Twelve S rRNA type-c (CAS 1627580-12-4) — is a 16-amino acid mitochondria-derived peptide (MDP) encoded within the 12S rRNA gene of the mitochondrial genome. It represents a newly recognized class of bioactive peptides that are translated from mitochondrial DNA and regulate nuclear gene expression, a phenomenon termed mitochondrial-nuclear cross-talk (retrograde signaling).
MOTS-c activates AMPK and inhibits the folate cycle to generate AICAR (ZMP), directly linking mitochondrial signaling to AMPK-mediated metabolic regulation. It translocates to the nucleus under metabolic stress conditions and modulates adaptive nuclear gene expression. Supplied as lyophilized peptide for non-clinical research use only.
COMMON RESEARCH APPLICATIONS
Mitochondrial-Nuclear Retrograde Signaling
MOTS-c translocates to the nucleus under stress, activating AMPK-target gene networks. Used in stress-challenge models (glucose deprivation, oxidative stress) to study mitochondrial-nuclear communication in HEK293, HepG2, and C2C12 cell lines.AMPK Activation via Folate Cycle Inhibition
MOTS-c inhibits the folate cycle, generating AICAR (ZMP) to activate AMPK. Used alongside direct AICAR and AMPK activators (metformin, A769662) to compare mitochondrial-origin vs. pharmacological AMPK activation in metabolic models.Metabolic Flexibility & Insulin Sensitivity Research
Studied for effects on glucose uptake, fatty acid oxidation, and mitochondrial biogenesis in skeletal muscle (C2C12) and adipocyte models; used in insulin resistance challenge systems alongside AICAR and resveratrol.Aging Biology & Longevity Research
Circulating MOTS-c levels decline with age. Used in senescence models to study age-related mitochondrial dysfunction, mitohormesis pathways, and the relationship between mitochondrial peptide signaling and cellular aging markers. -
Chemical Identity
Compound Name: MOTS-c
Synonyms: Mitochondrial ORF of the Twelve S rRNA-c · mitochondrial-derived peptide MOTS-c · MRPS12-encoded peptide
CAS Number: 1627580-12-4
EC Number: N/A (mitochondria-encoded peptide)
Molecular Formula: C₁₁₇H₂₀₈N₄₀O₃₃S
Molecular Weight: 2288.60 g/mol
Physical Properties
Appearance: White lyophilized powder
Solubility (water): ~1 mg/mL in sterile water or PBS (pH 7.4)
Solubility (organic): Limited; DMSO not recommended
Hygroscopic: Yes — store desiccated
Quality & Testing
Purity: ≥98% (HPLC)
Testing Method : Reverse-phase HPLC · MS confirmation · sequence verification
Verification
COA: Lot-specific, included with order (QR on label)
SDS Supplier : See SDS/MSDS · OSHA HCS / GHS compliant
Handling & Storage
Storage Temperature: Store desiccated at −20°C long-term; 2–8°C short-term. Protect from light. Refer to SDS/COA for compound-specific handling.
Light Sensitivity: Protect from light
Reconstitution: Dissolve in sterile water or PBS (pH 7.4) at ~1 mg/mL; aliquot and store at −80°C
Available Strengths: 10 mg
SKU (10 mg): VE-MOTS-010
-
-
What is this product?
MOTS-c is supplied as a lyophilized 16-amino acid mitochondria-derived peptide (CAS 1627580-12-4, MW 2288.60 g/mol) for laboratory research use only. It is encoded in mitochondrial DNA and represents a novel class of mitochondrial-origin bioactive peptides.
-
What purity and testing methods are used?
Each lot is tested by reverse-phase HPLC and MS confirmation to ≥98% purity with sequence verification. A lot-specific COA is included with every order, accessible via QR code on the label.
-
What are the key chemical identifiers?
CAS 1627580-12-4 · Molecular formula C₁₁₇H₂₀₈N₄₀O₃₃S · MW 2288.60 g/mol · Synonyms: Mitochondrial ORF of the Twelve S rRNA-c, MRPS12-encoded peptide, mitochondrial-derived peptide MOTS-c.
-
How is MOTS-c reconstituted for research use?
Dissolve in sterile water or PBS (pH 7.4) at ~1 mg/mL. Aliquot and store at −80°C for long-term stability. Avoid repeated freeze-thaw cycles. DMSO is not recommended.
-
What is the recommended storage upon receipt?
Store immediately at −20°C desiccated. For long-term stability, aliquot and store at −80°C. Short-term at 2–8°C is acceptable. Inspect vials for integrity upon receipt.
-
What is a COA and why does it matter?
A COA documents lot-specific purity (HPLC ≥98%), sequence verification (MS), and appearance. Each MOTS-c lot ships with a QR-accessible COA. Review before use to confirm identity and purity.
-
What is this product?
Reviews
There are no reviews yet.