AICAR

AICAR

Price range: $64.00 through $86.00

AICAR 50mg / 100mg

What is AICAR

AICAR is supplied strictly for laboratory research and analytical applications, commonly referenced in cellular aging, regeneration, and redox pathway studies.

  • Format: Lyophilized powder
  • Category: Anti-Aging & Healing
  • Documentation: COA available

Research Use Only — Not for Human Consumption.

SKU: N/A Category:

Description

  • About AICAR (Acadesine)

    AICAR — also known as Acadesine (CAS 2627-69-2) — is a cell-permeable nucleotide precursor and one of the most widely used pharmacological tools for studying AMP-activated protein kinase (AMPK) signaling in vitro and in preclinical models. It is supplied as a laboratory-grade lyophilized powder intended strictly for analytical and non-clinical research applications.

    Unlike exercise or caloric restriction, which activate AMPK by shifting the AMP:ATP ratio, AICAR is phosphorylated intracellularly to ZMP — an AMP mimetic that activates AMPK allosterically without depleting ATP. This makes it a uniquely clean pharmacological tool for isolating AMPK-dependent effects in cellular and preclinical models.

    COMMON RESEARCH APPLICATIONS

    AMPK Pathway Activation Studies
    Primary tool compound for AMPK activation via ZMP. Used in HepG2, C2C12, and HEK293 models at 0.5–2 mM to study ACC phosphorylation, mTORC1/S6K1 inhibition, and GLUT4 translocation as downstream AMPK effector endpoints.

    Mitochondrial Biogenesis Research
    AMPK-mediated PGC-1α activation downstream of ZMP accumulation. Used in skeletal muscle cell models (L6, C2C12) to study mitochondrial content (mtDNA copy number, TFAM expression) and biogenesis pathway mapping alongside AICAR vs. direct AMPK activators.

    Cancer Cell Metabolism & Apoptosis Screening
    Used in leukemia (Jurkat, Ramos), lymphoma, and solid tumor cell lines to study ZMP-mediated purine synthesis inhibition (AICARFT enzyme), metabolic stress induction, and apoptosis pathway activation independent of AMPK.

    Comparative AMPK Activator Benchmarking
    Reference standard for comparing ZMP-dependent vs. allosteric AMPK activators (A-769662, MK-8722, compound 991). Used in dose-response studies to characterize AMPK activation kinetics, isoform selectivity, and downstream pathway overlap.

  • Chemical Identity

    Compound Name: AICAR

    Synonyms: Acadesine · AICA-Riboside · NSC 105823 · 5-amino-1-β-D-ribofuranosyl-1H-imidazole-4-carboxamide

    CAS Number: 2627-69-2

    EC Number: 220-097-5

    Molecular Formula: C₉H₁₄N₄O₈P

    Molecular Weight: 338.21 g/mol

    Cayman Item No: 10010241

    Physical Properties

    Appearance: White to off-white lyophilized powder

    Solubility (water): ~2.5 mg/mL in PBS (pH 7.2)

    Solubility (organic): Soluble in ethanol, DMSO, DMF (~1–20 mg/mL)

    Hygroscopic: Yes — store desiccated Quality & Testing

    Quality & Testing

    Purity: Reverse-phase HPLC · MS confirmation · ICP copper verification

    Testing Method : Lot-specific, included with order (QR on label)

    Purity: ≥98% (HPLC)

    Testing Method: Reverse-phase HPLC · MS confirmation

    COA: Lot-specific, included with order (QR on label)

    SDS Supplier : Cayman Chemical Co., Ann Arbor MI

    SDS Revision: June 2025 · OSHA HCS / GHS compliant

    Handling & Storage

    Storage Temperature: Store desiccated at −20°C for long-term; 2–8°C short-term. Protect from light. Refer to SDS/COA for compound-specific handling.

    Light Sensitivity: Protect from light

    Reconstitution: Dissolve in PBS or DMSO; purge organic solvent with inert gas before use

    Available Strengths: 50 mg · 100 mg

    SKU (50 mg): VE-AICA-050

    SKU (100 mg): VE-AICA-100

    • What is this product?
      AICAR (Acadesine, CAS 2627-69-2, MW 338.21 g/mol) is supplied as a lyophilized cell-permeable nucleotide precursor and AMPK activator (via ZMP) for laboratory research use only. Available as VE-AICA-050 (50 mg) and VE-AICA-100 (100 mg).
    • What purity and testing methods are used?
      Each lot is tested by reverse-phase HPLC and MS confirmation to ≥98% purity. A lot-specific COA is included with every order. Scan the QR code on the label to access your lot documentation. SDS sourced from Cayman Chemical Co.
    • What are the key chemical identifiers?
      CAS 2627-69-2 · EC Number 220-097-5 · Molecular formula C₉H₁₄N₄O₈P (note: this is the 5-phosphorylated form; free nucleoside is C₉H₁₄N₄O₅) · MW 338.21 g/mol · Synonyms: Acadesine, AICA-Riboside, NSC 105823, 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside.
    • What is the mechanism by which AICAR activates AMPK?
      AICAR is phosphorylated intracellularly to ZMP (AICA-ribotide), a structural AMP mimetic. ZMP allosterically activates AMPK at the γ-subunit AMP-binding site without depleting ATP — making it a uniquely clean AMPK activator that decouples AMPK activation from cellular energy stress.
    • How is AICAR reconstituted for research use?
      Dissolve in PBS (pH 7.2) at ~2.5 mg/mL or DMSO at ~1–20 mg/mL. AICAR is hygroscopic — handle quickly and store desiccated. Purge organic solvents with inert gas before cell-based assays. Protect from light.
    • What is the recommended storage upon receipt?
      Store immediately at −20°C in a desiccated environment. Long-term storage at −80°C recommended for extended stability. Short-term 2–8°C acceptable for active use periods. Inspect vials for integrity upon receipt.
    • What is a COA and why does it matter?
      A COA documents lot-specific purity (HPLC ≥98%), MS identity confirmation, and appearance. Each AICAR lot ships with a QR-accessible COA from Cayman Chemical Co. Review before use to confirm identity and purity meet your experimental requirements.

Additional information

Dosage

50mg, 100mg

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